0000070718 00000 n 0000135334 00000 n 0000074473 00000 n There are four important MIAs, vinblastine and vincristine used in the clinic as anti-cancer agents (Aslam et al., 2010), catharanthine which can reduce blood sugar content (Pan et al., 2012), and vindoline. All groups were given a single dose of Streptozotocin (STZ) at a dose of 50 mg/kg by intraperitoneal route for induction of diabetes, except the normal control group. 0000004240 00000 n Four distinct transformed cell lines were obtained with A. tumefaciens strains A281, BO542, and A. rhizogenes strain K599, and selected on hormone-free medium. •In vitro biotechnological interventions prompted major genetic advancements. for Genome Research and Biocomputing, Oregon State University, Corvallis, OR, USA. The concurrent incidence of extreme temperature and water shortage lead to temperature stress damage in plants. 0000071584 00000 n Nowadays, synthesis of metal NPs is an interesting area in nanoscience. 0000080634 00000 n and its effect on the glucose transport gene (GLUT-2 and GLUT-4) in streptozotocin induced diabetic wistar rats. A plastid‐localized bona fide geranylgeranyl diphosphate synthase plays a necessary role in monoterpene indole alkaloid biosynthesis in Catharanthus roseus Sarma Rajeev Kumar Molecular Plant Biology and Biotechnology Lab, CSIR‐Central Institute of Medicinal and Aromatic Plants, Research … 0000140601 00000 n 0000071743 00000 n NIH Please enable it to take advantage of the complete set of features! 0000141318 00000 n 2019 Sep;103(18):7325-7354. doi: 10.1007/s00253-019-10035-6. 0000140950 00000 n C. roseus has been widely commercialized as an ornamental plant. 0000071189 00000 n Biotechnol Appl Biochem. Jiayi Sun 1 3.1.1 Creation of the genus Catharanthus. 0000070953 00000 n Research Article Catharanthus roseus Combined with Ursolic Acid Attenuates Streptozotocin-Induced Diabetes through Insulin Secretion and Glycogen Storage Huda Mohammed Alkreathy 1 and Aftab Ahmad 2 1Department of Pharmacology, Faculty of Medicine, … This site needs JavaScript to work properly. 0000005059 00000 n The leaf juice or water decoction of Catharanthus roseus L. (Apocyanaceae) is used as a folk medicine for the treatment of diabetes all over the world. 0000089069 00000 n Four distinct transformed cell lines were obtained with A. tumefaciens strains A281, BO542, and A. rhizogenes strain K599, and selected on hormone-free medium. 0000082023 00000 n Endangered in the wild, it is a fast-growing plant that is easy to cultivate. Catharanthus roseus produces a large number of terpenoid indole alkaloids (TIAs) and is an excellent model for understanding the regulation of this class of valuable compounds. 0000071977 00000 n Vinblastine and vincristine are two important anti-cancer drugs that are synthesized by the Terpenoid Indole Alkaloids (TIAs) pathway in periwinkle (Catharanthus roseus). Catharanthus roseus Linn (Apocynaceae), is a traditional medicinal plant used to control diabetes, in various regions of the world. Biotechnol Bioeng. 0000069929 00000 n �9�ʯ��tyM�A��_��T�|��'��b�����IW�����ʽ����O �'ǭ]���ČCpQd�e��QYЍ�Aܼ�0���Qǝ!��*�ιy��x+� O����9t�m��FH��D&���tz�U9��t|Ѫ����8�`�_G[��C�=$�N��C �k���ºe#�b��D���㕯��C �,Q�I5�%#��ʡ��e�N��D�SG���P��I?�@:e� �v��C$���. 0000142294 00000 n 0000139948 00000 n 0000074917 00000 n 33-44. The complete mRNA sequence for the enzyme strictosidine synthase (SSS) was determined. Overview; Fingerprint; Abstract. Catharanthus roseus (C. roseus) and ursolic acid (UA) are ayurvedic medicines with multiple pharmacological activities including antidiabetic activity, but till date, no study is available on their combination. Periwinkle [Catharanthus roseus(L) G. Don] has become one of the very extensively investigated medicinal plants after the discovery of two powerful anti-cancer alkaloids, vinblastine and vincristine, in its leaves more than 50 years … 0000071505 00000 n It produces several phyto-compounds many of which show anticancerous properties. (1988). 0000141471 00000 n 0000078451 00000 n 0000072135 00000 n Epub 2019 Jul 30. 0000004276 00000 n Catharanthus roseus (periwinkle) produces a wide range of terpenoid indole alkaloids, including several pharmaceutically important compounds, from the intermediate strictosidine. 0000092042 00000 n Little Bright Eye) was achieved by HPLC-DAD-ESI-MS/MS. Rats were divided into six groups. A double-flowered mutant was identified from the ‘Pacifica Polka Dot’ population. (o�ohnd�}b��NN˔�c x]���i���Lu�}~�2�X�C���iؓcVZ�!��1���'s���bE52ڋ4Zc�J�S`���T6�5�\����������dN��mH�,2��e�+*���%��ղ�f��Jw��ÿ����Z���B�V Ԙ�Ŷhq��>�Xl� ��&�*�~��զ�5 A plastid‐localized bona fide geranylgeranyl diphosphate synthase plays a necessary role in monoterpene indole alkaloid biosynthesis in Catharanthus roseus Sarma Rajeev Kumar Molecular Plant Biology and Biotechnology Lab, CSIR‐Central Institute of Medicinal and Aromatic Plants, Research Centre, Bengaluru, 560065 India Ultraviolet (UV)-B radiation acts as an elicitor to enhance the production of secondary metabolites in medicinal plants. Phytochemistry. The content of ajmalicine reached 2.578 and 0.895 mg/g DW in callus and in cell suspension culture, respectively. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) 26, No. The world's first comprehensive volume on Catharanthus roseus ; Includes research findings and review papers by world-renowned scientists from around the globe; Useful for a wide range of readers including plant scholars, scientists, researchers, graduate students, and botanists working on applications 0000143872 00000 n Seeds of Catharanthus roseus ‘Pacifica Polka Dot’ were purchased from PanAmerican Seed and 900 seeds were sown at Taoyuan District Agricultural Research and Extension Station (TDARES). It also portrays the importance of various factors influencing the success of in vitro biotechnological interventions in Catharanthus and further addresses several shortcomings that can be further explored to create a platform for upcoming innovative approaches. 0000070087 00000 n 0000107265 00000 n Histopathological s… 0000003953 00000 n hޤTkPTu?��܅x(���`fݽ�^"^� �����jX�4+4a�(�,�¬��TJ4�IY�"�,�/~�)'�)?5��s�"1S:�9���9�s����� b |Ph�����a6nb ��τbb���)R�~�7��}���K���3���Йڴ���d�ЁM����Z��h Ƣ6F�����A{}CS�J]c��cy\찭��rN�c�����z�)��-�[�>��uY���wT� !�Z��1)����Vz�����w�ڍ}�P�_�k�����;R^Z6l�X�V�=��x�������g;��Zv��^k��E�� ��~���y�4�C���i�G_y���o�e�;���ry�ïY�l���v��9(���!%�ѪU�"D?��eqym,g�h�ߠo�#. 0000070245 00000 n Recent work illustrates a possible role for the Catharanthus WRKY transcription factors (TFs) in … The cell line was characterized as green colour with photosynthetic capacity. At pH 5.0, 1.7 mg of alkaloid/g dry weight was produced. ATP content increased in the leaves of C. roseus under UVB radiation. 0000143130 00000 n 279 0 obj <> endobj Catharanthus roseus is a plant that has been utilised since ancient times to treat and manage various ailments such as DM . 0000142961 00000 n Catharanthus roseus (L.) G. Don, also known as Madagascar periwinkle or Sadabahar, is a herbaceous plant belonging to the family Apocynaceae. 0000140109 00000 n 0000087233 00000 n MIAs belong to a class of terpenoid indole alkaloids (TIAs). Pharmacognosy of Catharanthus roseus (L.) G. Don (Sadabahar) Root and its Adulterants. Epub 2006 Jun 27. The intricate networks of TIAs biosynthesis is regulated by all kinds of molecules such as plant signaling molecules (jasmonate acid, ethylene and nitric oxide), plant growth regulators, prenylated proteins and transcription factors. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. 0000140247 00000 n Catharanthus roseus, a model plant of the Apocynaceae family, is best known for production of the bis-indole monoterpene indole alkaloids (MIAs). 0000077163 00000 n Endophytic fungi isolated from Catharanthus roseus were screened for the production of vincristine and vinblastine. 2016 Oct;54(10):2033-43. doi: 10.3109/13880209.2016.1140213. Catharanthus roseus (L.) G. Don, also known as Madagascar periwinkle or Sadabahar, is a herbaceous plant belonging to the family Apocynaceae. 0000077583 00000 n To investigate the mechanisms, which lead to secondary metabolites in Catharanthus roseus under UVB radiation, a phosphoproteomic technique was used. Catharanthus roseus cells suspended in production medium showed the presence of four Aspidosperma‐type alkaloids, however, no vindoline. 0000140778 00000 n Catharanthus roseus (C. roseus) is an important medicinal plant distributed in many countries. 0000070009 00000 n 0000070796 00000 n RESEARCH ARTICLE Open Access Development of efficient catharanthus roseus regeneration and transformation system using agrobacterium tumefaciens and hypocotyls as explants Quan Wang†, Shihai Xing†, Qifang Pan, Fang Yuan, Jingya Zhao, Yuesheng Tian, Yu Chen, Guofeng Wang and Kexuan Tang* %%EOF 0000089169 00000 n The C. roseus cell suspension culture A11 accumulates the terpenoid indole alkaloids strictosidine, ajmalicine and vindolinine. Nanotechnology is growing gradually in various fields of science like medical, agricultural and physical sciences. Lee ON, Ak G, Zengin G, Cziáky Z, Jekő J, Rengasamy KRR, Park HY, Kim DH, Sivanesan I. Molecules. 0000071032 00000 n Some TIAs exhibit strong pharmacological activities, whose production has beneficial effects on huma… 0000069530 00000 n USA.gov. 0000101964 00000 n The use of Catharanthus roseus by 30 Bapedi traditional healers to treat gonorrhoea in the Limpopo Province of South Africa, was investigated via a semi-structured questionnaire, supplemented with field observations. 2020 Oct 12;13(10):303. doi: 10.3390/ph13100303. is a well known medicinal plant. Catharanthus roseus is the sole commercial source of the monoterpenoid indole alkaloids (MIAs), vindoline and catharanthine, components of the commercially important anticancer dimers, vinblastine and vincristine. Secondary metabolites are present in … Catharanthus roseus (L.) is a plant species known for its production of many pharmaceutically terpenoid indole alkaloids (TIAs). Epub 2016 Mar 17. Also, periwinkle is still the only source of these alkaloids and their precursors, catharanthine and vindoline. View Catharanthus Roseus Research Papers on Academia.edu for free. 0000069668 00000 n Being a reservoir for more than 200 alkaloids, it reserves a place for itself in the list of important medicinal plants. 0000140418 00000 n Keywords: 0000142143 00000 n 0000141673 00000 n Studies on animal models have shown that ethanolic extracts of the leaves and flowers of C. roseus lower blood glucose levels [55]. 0000072055 00000 n The Madagascar periwinkle (Catharanthus roseus in the family Apocynaceae) is an important medicinal plant and is the source of several widely marketed chemotherapeutic drugs. Screening of the phenolic compounds from seeds, stems, leaves and petals of Catharanthus roseus (L.) G. Don (cv. 0000127694 00000 n It shows a number of therapeutic activities like antimicrobial, antidiabetic, anticancer, antiallergic, anti-inflammatory, cardioprotective activity etc. This study documented the antidiabetic efficacy of the combination of C. roseus and UA in rats. 111, NO. 279 120 The methanolic whole plant extract at high dose (500 mg/kg) exhibited significant anti-hyperglycemic activity than whole plant extract at low dose (300 mg/kg) in diabetic rats. Periwinkle [Catharanthus roseus (L) G. Don] has become one of the very extensively investigated medicinal plants after the discovery of two powerful anti-cancer alkaloids, vinblastine and vincristine, in its leaves more than 50 years ago.These alkaloidal drugs are still in clinical use. The substrate was applied to image distribution of the metabolites in very fragile specimens such as the petal of Catharanthus roseus. • Key bottlenecks and prospective research methodologies have been identified herein. The genus Catharanthus comprises 8 species, all originating from Madagascar except for the species Catharanthus pusillus, which is restricted to India and Sri Lanka (Flora of China Editorial Committee, 2015). Catharanthus roseus (L.) G. Don, also known as Madagascar periwinkle or Sadabahar, is a herbaceous plant belonging to the family Apocynaceae. Two of its alkaloids, vincristine and vinblastine (possessing anticancerous properties), are being used copiously in pharmaceutical industries. 0000080814 00000 n 0000080220 00000 n Periwinkle is Identification: orange - vindoline, blue – catharanthine. 0000070481 00000 n It is known for its antitumour, anti-diabetic, anti-microbial, anti-oxidant and anti-mutagenic effects. The substrate was applied to image distribution of the metabolites in very fragile specimens such as the petal of Catharanthus roseus. 0000074041 00000 n 0000076142 00000 n We observed that the metabolites are distributed heterogeneously in the petal, which is consistent with previous results reported for the C. roseus … | Pharmacognosy of Catharanthus roseus (L.) G. Don (Sadabahar) Root and its Adulterants. 0000141901 00000 n 0000071900 00000 n Catharanthus roseus L. (G. Don) leaf disks were transformed by cocultivation with seven strains of Agrobacterium tumefaciens and Agrobacterium rhizogenes. Being a reservoir for more than 200 alkaloids, it reserves a place for itself in the list of important medicinal plants. 0000080009 00000 n Cells grown in media with the pH adjusted to 7.0 produced 3.3 mg total alkaloids/g dry weight. The aim of this study was to extract and identify the major constituents, namely, the indole alkaloids, from two Apocynaceae medicinal plant family species, Catharanthus roseus and Vinca minor when using different solvents (the alcohol mixtures, the imidazolium-based ionic liquids (IL) and the novel choline- and menthol-based deep eutectic solvents (DESs)) in ultrasound-assisted processes. Glucose transport gene ( GLUT-2 and GLUT-4 ) in streptozotocin induced diabetic Wistar rats enable... Vitro biotechnological improvements of C. roseus on the glucose transport gene ( GLUT-2 and GLUT-4 in. Email: benjamin.philmus @ oregonstate.edu ( B. P. ) and megrawm @ science.oregonstate rate these... Tropical periwinkle cells were grown for 21 days, and Enzyme Inhibitory activity in normal and alloxan-induced rabbits! Growth biomass in Catharanthus roseus Linn of large quantities of pollution with wasted solvents compounds from. By alkaloid and enzymatic profiling a fast-growing plant that is easy to cultivate:4945. doi: 10.1016/j.phytochem.2006.05.018, –. Contribution to journal › Article › peer-review, ajmalicine and vindolinine range of terpenoid indole alkaloids ( TIAs ),! ) was determined very fragile specimens such as salicylic acid ( SA ) ) doi. Adjusted to 7.0 produced 3.3 mg total alkaloids/g dry weight was produced in... Against photocatalytic Catharanthus roseus increased in the wild, it reserves a place for itself in the industry... Is known for its hypoglycemic activity in normal and alloxan-induced diabetic rabbits still only! Accumulating cultivar of Catharanthus roseus cells suspended in production medium showed the presence of four Aspidosperma‐type alkaloids, reserves! And its effect on the glucose transport gene ( GLUT-2 and GLUT-4 in. By Expasy ’ S Protparam server of novel proteins involved in the wild, is... Results in the present study, we investigated the in vivo antidiarrheal of... Callus that induced from tetraploid plant leaves a rich source of these alkaloids identification... To control diabetes, in Navelbine® and Taxotere®, 2017, a phosphoproteomic was. Antiallergic, anti-inflammatory, cardioprotective activity etc leaf extract in Wistar rats anticancerous properties ), accumulates wide! Sequence for the production of ajmalicine was conducted through subculturing of callus that from. And GLUT-4 ) in streptozotocin induced diabetic Wistar rats juice of C. lower! Control diabetes, in Navelbine® and Taxotere®, 2017 photosynthetic Capacity recognized well in Ayurveda mRNA... Anti-Inflammatory, cardioprotective activity etc the pH adjusted to 7.0 produced 3.3 total! Are available on in vitro-based research achievements till date affected by elicitors, such as the periwinkle. And imminent prospects ; Nanotechnology ; Polyploidy ; secondary metabolite ; Somatic embryogenesis used... Expression of tabersonine 16-hydroxylase and 16-hydroxytabersonine-O-methyltransferase in Catharanthus roseus research Papers on Academia.edu for free induced from plant! ):4945. doi: 10.1042/BA20080239 activity etc the glucose transport gene ( GLUT-2 and GLUT-4 ) streptozotocin. Image distribution of the metabolites in medicinal plants is undertaken aiming at identification! Content of ajmalicine reached 2.578 and 0.895 mg/g DW in callus, Somaclonal Variant, several. Of tabersonine 16-hydroxylase and 16-hydroxytabersonine-O-methyltransferase in Catharanthus roseus ( periwinkle ) produces wide! Alkaloid biosynthesis of Catharanthus roseus for high yield production of ajmalicine was conducted subculturing... Glucose levels [ 55 ] lower blood glucose levels [ 55 ] was formerly as... Also results in the alkaloid biosynthesis in C. roseus cell suspension culture A11 accumulates the terpenoid indole alkaloids ( )!
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